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Date Published: 5/15/2012, Issue 63; doi: 10.3791/4112 Keywords: Chemistry, Issue 63, bis-peptides, solid phase peptide synthesis, bis-amino acids, safety catch, HMBA, DTRAIn 1962, R. Merrifield published the first procedure using solid-phase peptide synthesis as a novel route to efficiently synthesize peptides. The primary challenge in solid-phase synthesis is diagnosing synthetic progress and problem solving since no intermediates are isolated. This que quickly proved advantageous over its solution-phase predecessor in both time and labor. This has lead to the development of many colorimetric tests including those to identify if free amines (Kaiser Test is not generally applicable in our solid-phase synthesis due to the almost exclusive use of secondary amines or amines attached to a quaternary carbon.
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